Molecular Diagnostics

Special Information for Microbiology Submissions

Sample preparation fee will apply for submissions to the Microbiology Laboratory that are too large to process in the Microbiology Laboratory. The fees are as follows:

Sample that requires trim down for processing in the Microbiology lab (e.g., liver or placenta section submitted to Micro that is too large to process in that lab) - $30.00.

Sample that requires tissue collection for Microbiology testing that requires consultation with the pathologist such as collecting samples from a whole piglet or taking a joint swab from a leg - $50.00.


PCR Submissions


PDS is no longer accepting swabs in gel media for PCR testing. Refer to PDS is no longer accepting Gel Swabs for PCR Testing.

A minimum of 5 grams of feces is required for Johne’s PCR testing. Samples under 2 grams will not be processed, client will be notified, and additional feces must be submitted.

Tritrichomanas samples require a 48-hour incubation at 35OC prior to processing. Please indicate on the submission form if sample was incubated prior to shipping. The test turnaround time does not include the incubation time, please add 2 days to turnaround time if the sample was not incubated before shipping.

If you are submitting samples for Bacteriology as well as PCR, submit a gel media swab (preferred for culture) as well as a swab that is acceptable for PCR.

If urgent results are required, the submission form must be marked as STAT. Please contact the lab when a STAT sample will be submitted. Short turnaround times cannot be guaranteed, especially for large numbers of samples. STAT samples must arrive in the lab by 10 A.M., overtime charges may apply for samples arriving after 10 A.M. STAT testing may not be available for all PCR tests.

PCR STAT requests:

STAT PCR test options are available for the most requested STAT tests (PRRS, Porcine Corona Panel, Mycoplasma hyopneumoniae, Equine Respiratory Panel, Equine herpesvirus 1 and 4, refer to Test section of this guide. Price includes the STAT fee.

For all other PCR STAT requests, the STAT charge is applied as a percentage of the test cost per test ordered. The STAT charge is discounted if multiple samples are submitted.

Example pricing:

• Sample 1-2 at 150% (e.g., test cost is $50.00, one sample STAT is $75.00)
• Samples 3-9 at 130% (e.g., test cost is $50.00, 3 samples STAT is $195.00)
• More than 10 samples at 110% (e.g., test cost is $50.00, 10 samples STAT is $550.00)

Not all PCR tests are available STAT. Refer to STAT Submissions for a list of tests NOT available STAT.

Samples must arrive in the lab by 10 A.M. Additional charges will apply for late arriving samples if overtime is required. Contact PDS to discuss overtime charges prior to submitting late samples for same day testing.

Tests that require bacterial culture for extraction will have longer turnaround times. These samples will be forwarded to the bacteriology section for culture before they can be submitted to the PCR section for testing.

For large submissions of Mycobacterium paratuberculosis (Johne’s disease), and Bovine Viral Diarrhea (BVD), turnaround times will vary according to the number of samples submitted. Please contact the lab for estimated turnaround times.

Polymerase Chain Reaction (PCR) Reference Guide

African Swine Fever (CFIA Accredited Test)
Avian Influenza Virus (CFIA Accredited Test)
Avian Influenza Virus H5; H7
Avian paramyxovirus-1 (CFIA Accredited Test)
Bacterial Identification by Sequencing
Bovine Coronavirus
Bovine Diarrhea Panel
Bovine Herpesvirus-1 (BHV-1)
Bovine Papilloma Virus
Bovine Parainfluenza 3 Virus
Bovine Respiratory Syncytial Virus (BRSV)
Bovine Respiratory Panel (6 or 7 targets)
Bovine Rotavirus
Bovine Viral Diarrhea Virus (BVDV)
Brachyspira hyodysenteriae and pilosicoli
Campylobacter fetus ssp. fetus
Campylobacter fetus ssp. venerealis

Campylobacter sp.
Chlamydophilia abortus
Chlamydophilia psittaci

Classical Swine Fever Virus (CFIA Accredited Test)
Clostridium difficile Genotyping
Clostridium perfringens genotyping
Coxiella burnetti
CWD Genotyping – White tailed Deer, Elk
E. coli virulence factors – Bovine, Porcine, Canine Panels
Equine Herpesvirus (EHV) types 1 & 4
Equine Herpesvirus 1 subtyping
Equine Respiratory Panel
Feline Calicivirus
Feline Herpes Virus
Flax CDC Triffid
Foot and Mouth Disease (CFIA Accredited Test)
Gallid Herpesvirus (ILT)

Please refer to the specific agent in the test section of this guide for prices and details. If agent not listed above may be available as a referred-out test or a new test (contact PDS).

Glaesserella parasuis
Herpesvirus sp.
Infectious Bovine Rhinotracheitis Virus (IBR)
Influenza A Virus, in all species
Influenza D Virus
Lawsonia intracellularis
Malignant Catarrhal Fever (MCF)/Ovine Herpes Virus 2
Mycobacterium sp. (Speciation can be done by sequencing)
Mycobacterium avium spp. paratuberculosis (Johne’s Disease)
Mycoplasma bovis
Mycoplasma gallisepticum / synoviae

Mycoplasma haemofelis, haemominutum
Mycoplasma hyopneumoniae

Mycoplasma sp. Identification by Gene Sequencing
Mycoplasma hyosynoviae
Mycoplasma hyorhinis
Mycoplasma ovipneumoniae

Pasteurella multocida tox A detection
Porcine Circovirus-2
Porcine Circovirus-3
Porcine Corona Panel (PEDV, TGEV, pdCOV)
Porcine Diarrhea Panels
Porcine Epidemic Diarrhea Virus
Porcine Parvovirus
Porcine Rotovirus, group specific (A, B, or C)
Porcine Sapovirus
Seneca A Virus
Streptococcus equi ssp. equi
Streptococcus equi ssp. zooepidemicus

Swine Influenza Typing – H1, H3
Transmissible Gastroenteritis Virus (TGEV)
Tritrichomonas fetus
Ureaplasma diversum

West Nile Virus