Histopathological Stains
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Acid orcein giemsa - elastic fibers, mast cell granules, bacteria
Alcian Blue - acidic mucins
Alcian Blue/PAS - acidic, neutral mucin
Alizarin Red S - Calcium
Aluminum stain (Morin) - aluminum
Congo Red - amyloid
Duffy's - mast cell and eosinophils
Fites - Mycobacterium and Nocardia sp.
Fontana Masson - melanin, late lipofuscins, argentaffin cells
Formalin Pigment Removal
Giemsa - mast cell granules, bacteria
Gordon and Sweet's - reticulin fibers
Gram - bacteria
Grimelius' - neuroendocrine cells
Grocott's Methenamine Silver - fungus
Grocott's Methenamine Silver/H&E - fungus
Hall's - bile
Hematoxylin and Eosin (H&E) - routine stain
Hematoxylin Orcein Phloxine Saffron (HOPS) - muscle, collagen, elastic fibers
Holmes - nerve fibers
Luxol Fast Blue/Cresyl Violet - myelin, nissl substance
Luxol Fast Blue/H&E - myelin
Macchiavello's rickettsia
Masson's Trichrome - muscle and collagen fibers Mercury pigment removal
Melanin Bleach/H&E
Methyl Green Pyronine - plasma cells
Oil Red O - lipids – (Note: please submit wet tissue)
Okajima's - hemoglobin
Periodic Acid Methenamine Silver - basement membranes
Periodic Acid Schiff's (PAS) - neutral mucins, glycogen, fungus
Periodic Acid Schiff's/Diastase - glycogen
Perl's Prussian Blue - hemosiderin
Phosphotungstic Acid Hematoxylin (PTAH) - fibrin, muscle striations
Rhodanine - copper
Safranine O/Fast Green - cartilage
Schmorl's - lipofuscins, melanin
Sirius Red (Picrosirus) - osteoid
Toluidine Blue - Mast cells
Von Kossa - calcium salts
Warthin Faulkner - spirochetes, bacteria
Weigert Van Gieson's - muscle, collagen, and elastic fibers
Ziehl Neelsen - Mycobacterium sp.