Special Information for Microbiology Submissions
Sample preparation fee:
Tissue Size Guidelines:
- Limit tissue size to the size of a human palm. Bloody or oversized samples may delay processing and result in additional charges.
Additional Charges:
- Tissue samples that are too large to process in the laboratory (e.g. liver, placenta): $52.50.
- Tissue samples that require consultation with the pathologist such as collecting samples from a whole piglet or taking a joint swab from a leg: $84.00.
PCR Submissions
PDS is no longer accepting swabs in gel media for PCR testing. Refer to PDS is no longer accepting Gel Swabs for PCR Testing.
A minimum of 5 grams of feces is required for Johne’s PCR testing. Samples under 2 grams will not be processed, the client will be notified, and additional feces must be submitted. Refer to Johne’s Testing at PDS found under Resources->Submission Protocols.
BVD Skin Testing: The BVD Skin Biopsy IHC test is no longer available. It has been replaced by BVD Real Time PCR, which requires fresh skin/ear notches. For further details, refer to BVD Sampling Instructions found under Resources->Bovine on the PDS website (pdsinc.ca).
There are 2 test options available:
- Individual testing – individual samples are analyzed, and a result will be provided for each individual animal, price listed is per animal.
- Pooled testing – 2 to 10 samples are pooled into one sample, the pooled sample is analyzed, and the result will be for the pooled sample (not individual results for each animal), price listed is per pool.
Additional charges will apply if you request that BVD positive pools be tested individually, contact PDS for current pricing.
Tritrichomanas samples require a 48-hour incubation at 35OC prior to processing. Please indicate on the submission form if sample was incubated prior to shipping. The test turnaround time does not include the incubation time, please add 2 days to turnaround time if the sample was not incubated before shipping.
When submitting samples for Bacteriology as well as PCR, submit a gel media swab (preferred for culture) as well as a swab that is acceptable for PCR.
If urgent results are required, the submission form must be marked as STAT. Please contact the lab when a STAT sample will be submitted. Short turnaround times cannot be guaranteed, especially for large numbers of samples. STAT samples must arrive in the lab by 10 A.M., overtime charges may apply for samples arriving after 10:00 A.M. STAT testing is not available for all PCR tests – refer to Stat Submissions -> Tests Excluded from STAT Testing.
PCR STAT requests
STAT PCR test options are available for the most requested STAT tests (PRRS, Porcine Corona Panel, Mycoplasma hyopneumoniae, Equine Respiratory Panel, Equine herpesvirus 1 and 4), refer to Test section of PDS Test and Services Guide. Price includes the STAT fee.
For all other PCR STAT requests, the STAT charge is applied as a percentage of the test cost per test ordered. The STAT charge is discounted if multiple samples are submitted.
Example pricing:
- Sample 1-2 at 150% (e.g., test cost is $50.00, one sample STAT is $75.00)
- Samples 3-9 at 130% (e.g., test cost is $50.00, 3 samples STAT is $195.00)
- More than 10 samples at 110% (e.g., test cost is $50.00, 10 samples STAT is $550.00)
Not all PCR tests are available STAT. Refer to STAT Submissions for a list of tests NOT available STAT.
Samples must arrive in the lab by 10 A.M. Additional charges will apply for late arriving samples if overtime is required. Contact PDS to discuss overtime charges prior to submitting late samples for same day testing.
Tests that require bacterial culture and PCR will have longer turnaround times. These samples will be forwarded to the bacteriology section for culture before they can be submitted to the PCR section for testing.
For large submissions of Mycobacterium paratuberculosis (Johne’s disease), and Bovine Viral Diarrhea (BVD), turnaround times will vary according to the number of samples submitted. Please contact the lab for estimated turnaround times.
Polymerase Chain Reaction (PCR) Reference Guide
Click to expand
- African Swine Fever (CFIA Accredited Test)
- Anthrax
- Atypical Porcine Pestivirus PCR (APPV)
- Avian Adenovirus
- Avian Influenza Virus (CFIA Accredited Test)
- Avian Influenza Virus H5; H7
- Avian metapneumoniae virus
- Avian paramyxovirus-1 (CFIA Accredited Test)
- Bacterial Identification by Sequencing
- Bovine Coronavirus
- Bovine Diarrhea Panel
- Bovine Herpesvirus-1 (BHV-1)
- Bovine Papilloma Virus
- Bovine Parainfluenza 3 Virus
- Bovine Respiratory Syncytial Virus (BRSV)
- Bovine Respiratory Panel (6 or 7 targets)
- Bovine Rotavirus
- Bovine Viral Diarrhea Virus (BVDV)
- Brachyspira hyodysenteriae and pilosicoli
- Cache Valley Virus
- Campylobacter fetus ssp. fetus
- Campylobacter fetus ssp. venerealis
- Campylobacter sp.
- Chlamydophilia abortus
- Chlamydophilia psittaci
- Classical Swine Fever Virus (CFIA Accredited Test)
- Clostridium difficile Genotyping
- Clostridium perfringens genotyping
- Coxiella burnetii
- CWD Genotyping – White tailed Deer, Elk
- E. coli virulence factors – Bovine, Porcine, Canine Panels
- Equine Herpesvirus (EHV) types 1 & 4
- Equine Herpesvirus 1 subtyping
- Equine Respiratory Panel
- Feline Calicivirus
- Feline Herpes Virus
- Flax CDC Triffid
- Foot and Mouth Disease (CFIA Accredited Test)
- Gallid Herpesvirus (ILT)
- Glaesserella parasuis
- Herpesvirus sp.
- Infectious Bovine Rhinotracheitis Virus (IBR)
- Influenza A Virus, in all species
- Influenza D Virus
- Lawsonia intracellularis
- Malignant Catarrhal Fever (MCF)/Ovine Herpes Virus 2
- Mycobacterium sp. (Speciation can be done by sequencing)
- Mycobacterium avium spp. paratuberculosis (Johne’s Disease)
- Mycoplasma bovis
- Mycoplasma gallisepticum/synoviae
- Mycoplasma haemofelis, haemominutum
- Mycoplasma hyopneumoniae
- Mycoplasma sp. Identification by Gene Sequencing
- Mycoplasma hyosynoviae
- Mycoplasma hyorhinis
- Mycoplasma ovipneumoniae
- Neospora caninum
- Pasteurella multocida tox A detection
- Porcine Astrovirus Type 3 PCR (PAstV3)
- Porcine Astrovirus Type 4 PCR (PAstV4)
- Porcine Circovirus-2
- Porcine Circovirus-3
- Porcine Corona Panel (PEDV, TGEV, pdCOV)
- Porcine Diarrhea Panels
- Porcine Epidemic Diarrhea Virus
- Porcine Parainfluenza Virus Type 1 PCR
- Porcine Parvovirus
- PRRS
- Porcine Rotavirus, group specific (A, B, or C)
- Porcine Sapelovirus PCR
- Porcine Sapovirus
- Porcine Teschovirus PCR (PTV)
- Seneca A Virus
- Streptococcus equi ssp. equi
- Streptococcus equi ssp. zooepidemicus
- Swine Influenza Typing – H1, H3
- Transmissible Gastroenteritis Virus (TGEV)
- Tritrichomonas fetus
- Ureaplasma diversum
- West Nile Virus
Please refer to the specific agent in the test section of this guide for prices and details. If agent not listed above may be available as a referred-out test or a new test (contact PDS).