Molecular Diagnostics

Special Information for Microbiology Submissions


Sample preparation fee will apply for submissions to the Microbiology Laboratory that are too large to process in the Microbiology Laboratory. The fees are as follows:

a. Sample that requires trim down for processing in the Microbiology lab (e.g., liver or placenta section submitted to Micro that is too large to process in that lab) - $30.00.

b. Sample that requires tissue collection for Microbiology testing that requires consultation with the pathologist such as collecting samples from a whole piglet or taking a joint swab from a leg - $50.00.

 

PCR Submissions

 

Gel Swabs are not acceptable for PCR Testing - additional information.

  • If you are submitting samples for Bacteriology as well as PCR, submit a gel media swab (preferred for culture) as well as a swab that is acceptable for PCR.

If urgent results are required, the submission form must be marked as “STAT”. Please contact the lab when a “STAT” sample will be submitted. Short turnaround times cannot be guaranteed, especially for large numbers of samples. STAT samples must arrive in the lab by 10 A.M., overtime charges may apply for samples arriving after 10 A.M.
  
For PCR STAT requests the STAT charge is now applied as a percentage of the test cost per test ordered. The STAT charge is discounted if multiple samples are submitted.

Example Pricing:

Sample 1 - 2 at 150% (e.g., test cost is $50.00, one sample STAT is $75.00).

Sample 3 - 9 at 130% (e.g., test cost is $50.00, 3 samples STAT is $195.00).

More than 10 samples at 110% (e.g., test cost is $50.00, 10 samples STAT is $550.00)

New PCR STAT tests have been created for the tests most commonly requested STAT (PRRS, Porcine Corona Panel, Mycoplasma hyopneumoniae, Equine Respiratory Panel, Equine herpesvirus 1 and 4, refer to Test list. These tests include the STAT fee.

Not all PCR tests are available STAT. Contact PDS Diagnostic Services Office (306-966-7316) to confirm if STAT testing is available.

Samples must arrive in the lab by 10 A.M. Additional charges will apply for late arriving samples if overtime is required. Contact PDS to discuss overtime charges prior to submitting late samples for same day testing.

Tests that require bacterial culture for extraction will have longer turnaround times. These samples will be forwarded to the bacteriology section for culture before they can be submitted to the PCR section for testing.

For large submissions of Mycobacterium paratuberculosis (Johne’s disease), and Bovine Viral Diarrhea (BVD), turnaround times will vary according to the number of samples submitted. Please contact the lab for estimated turnaround times.

Polymerase Chain Reaction (PCR) Reference Guide

Anthrax 
Avian Influenza virus (CFIA Accredited Test)
Avian Influenza virus H5; H7
Avian paramyxovirus-1 (CFIA Accredited Test)
Bacterial Identification by Sequencing
Bovine Coronavirus
Bovine Herpesvirus -1 (BoHV-1)
Bovine Papilloma virus
Bovine Parainfluenza -3
Bovine Respiratory Syncytial Virus (BRSV)
Bovine Respiratory Panel (5 or 6 targets)
Bovine Rotavirus
Bovine Viral Diarrhea Virus (BVDV)
Brachyspira hyodysenteriae and pilosicoli
Campylobacter sp.
Campylobacter fetus ssp. fetus
Campylobacter fetus ssp. venerealis
Chlamydia abortus
Chlamydia psittaci
Classical Swine Fever Virus (CFIA Accredited Test)
Clostridioides difficile Genotyping
Clostridium perfringens genotyping
Coxiella burnetii
E. coli O157:H7
E. coli virulence factors Bovine, Porcine, Canine panels
Porcine Parvovirus
Equine Herpesvirus (EHV) types 1 & 4
Equine Influenza virus
Equine Respiratory Panel
Flax CDC Triffid
Foot and Mouth Disease (CFIA Accredited Test) .
Francisella tularensis
Haemophilus parasuis


Please refer to the specific agent in the test section of this guide for prices and details. If agent not listed above may be available as a referred-out test or a new test (contact PDS).

Herpesvirus sp.
Infectious Bovine Rhinotracheitis virus (IBR)
Influenza A virus, in all species
Lawsonia intracellularis
Malignant Catarrhal Fever (MCF)
Mycobacterium sp. (Speciation can be done by sequencing)
Mycoplasma sp. (Speciation can be done by sequencing)
Mycobacterium avium spp. avium
Mycobacterium avium spp. paratuberculosis (Johne’s Disease)
Mycobacterium bovis
Mycoplasma bovis
Mycoplasma gallisepticum / synoviae
Mycoplasma haemofelis, haemominutum
Mycoplasma hyopneumoniae
Mycoplasma sp. Identification by Gene Sequencing
Mycoplasma hyosynoviae
Mycoplasma hyorhinis
Mycoplasma ovipneumoniae
Bovine Parainfluenza 3 Virus (BPIV3)
Pasteurella multocida tox A detection
Porcine Circovirus-2
Porcine Circovirus-3
Porcine Corona Panel (PEDV, TGEV, pdCov)
Porcine Epidemic Diarrhea Virus
PRRS
Porcine Rotavirus, group specific (A, B, or C)
Streptococcus equi
Swine Influenza Virus, H1, H3
Transmissible Gastroenteritis Virus (TGEV)
Tritrichomonas fetus
Ureaplasma sp.
Ureaplasma diversum
West Nile Virus