Histopathological Stains

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Acid orcein giemsa - elastic fibers, mast cell granules, bacteria

Alcian Blue - acidic mucins

Alcian Blue/PAS - acidic, neutral mucin

Alizarin Red S - Calcium

Aluminum stain (Morin) - aluminum

Congo Red - amyloid

Duffy's - mast cell and eosinophils

Fites - Mycobacterium and Nocardia sp.

Fontana Masson - melanin, late lipofuscins, argentaffin cells

Formalin Pigment Removal

Giemsa - mast cell granules, bacteria

Gordon and Sweet's - reticulin fibers

Gram - bacteria

Grimelius' - neuroendocrine cells

Grocott's Methenamine Silver - fungus

Grocott's Methenamine Silver/H&E - fungus

Hall's - bile

Hematoxylin and Eosin (H&E) - routine stain

Hematoxylin Orcein Phloxine Saffron (HOPS) - muscle, collagen, elastic fibers

Holmes - nerve fibers

Luxol Fast Blue/Cresyl Violet - myelin, nissl substance

Luxol Fast Blue/H&E - myelin

Macchiavello's rickettsia

Masson's Trichrome - muscle and collagen fibers Mercury pigment removal

Melanin Bleach/H&E

Methyl Green Pyronine - plasma cells

Oil Red O - lipids – (Note: please submit wet tissue)

Okajima's - hemoglobin

Periodic Acid Methenamine Silver - basement membranes

Periodic Acid Schiff's (PAS) - neutral mucins, glycogen, fungus

Periodic Acid Schiff's/Diastase - glycogen

Perl's Prussian Blue - hemosiderin

Phosphotungstic Acid Hematoxylin (PTAH) - fibrin, muscle striations

Rhodanine - copper

Safranine O/Fast Green - cartilage

Schmorl's - lipofuscins, melanin

Sirius Red (Picrosirus) - osteoid

Toluidine Blue - Mast cells

Von Kossa - calcium salts

Warthin Faulkner - spirochetes, bacteria

Weigert Van Gieson's - muscle, collagen, and elastic fibers

Ziehl Neelsen - Mycobacterium sp.